engleski

Characteristics of early-medievnal inhabitants of Naklice (southern Croatia) revealed by mytochondrial DNA analysis

In recent years, anthropology has been using modern genetic analysis in order to elucidate kinship inside ancient populations and to clarify pathways of their migrations and origin. Croatia has numerous archaeological sites from the early Middle Ages which have not been genetically analyzed. Excavation of 19 ancient graves at Naklice (Southern Croatia) has provided unique opportunity to investigate the gene pool ethnogenesis of ancient Southern Croatian population. The aim of this study was to determine the hypervariable region 1 (HV1) sequence of the ancient DNA extracted from the Naklice burial site skeletons and to assign them to appropriate haplogroups. Ancient DNA was extracted from skeletons’ teeth/bones found in a graveyard. All samples were analyzed to determine the autosomal short tandem repeats (STR) profile of each skeleton. This was accomplished by sequencing the hypervariable region 1 (HV1) of the mitochondrial control DNA. Mitochondrial HV1 DNA region was successfully amplified from 27 out of 35 skeletal remains. Each of the 27 mtDNAs was compared to the Cambridge Reference Sequence and was assigned to an appropriate haplogroup. Haplogroup assignment revealed that 18 haplotypes belonged to haplogroup H (67 %), 5 (18%) belonged to haplogroup J, 3 (11%) belonged to haplogroup U5 and 1 (4 %) belonged to haplogroup HV. The frequency of haplogroups differs from the frequency of the same haplogroups in living population of Croatia.

Southern Croatian; early medieval population; ancient DNA; mtDNA; HV1

nije evidentirano