engleski

Crystallization and preliminary X-ray studies of purine nucleoside phosphorilase from cellulomonas sp.

The commercially available enzyme purine nucleoside phosphorylase (PNP) from Cellulomonas sp. was purified by ion-exchange chromatography, partially sequenced and crystallized in two different crystal forms using the hanging-drop vapour-diffusion technique. Crystal form A grows as polyeders and/or cubes in the cubic space group P4(2)32 with unit-cell dimension a = 162.5 Angstrom. Crystal form B appears as thick plates in the space group P2(1)2(1)2(1) with unit-cell dimensions a = 63.2, b = 108.3 and c = 117.4 Angstrom. Both crystal forms contain three monomers (one trimer) in the asymmetric unit.

Crystallization

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