engleski

Inhibitory effects of extracts from the marine alga Caulerpa taxifolia and of toxin from Caulerpa racemosa on multixenobiotic resistance in the marine sponge Geodia cydonium

The invasive growth of the introduced green alga Caulerpa taxifolia, already affecting the richness and diversify of the littoral ecosystems, has become a major ecological problem in the Mediterranean Sea. Previously, we demonstrated that the water pollutant tributyltin induces apoptosis in tissue of the marine sponge Geodia cydonium at concentrations of 3 mu M and higher. Here we show that exposure of G. cydonium to low (non-toxic) concentrations of Cauierpa extract or purified caulerpin (10 mu g/ml) together with low doses of tributyltin (1 mu M; non-toxic), results in a strong apoptotic effect. Evidence is presented that the enhancement of toxicity of tributyltin by Caulerpa extract is at least partially caused by inhibition of the multixenobiotic resistance (MXR)pump by the algal toxin. Caulerpa extract, as well as caulerpin, strongly enhance the accumulation of the test substrate of MXR, rhodamine B, in the gills of the mussel Dreissena polymorpha, used as a model system for testing MXR-inhibiting potential.

apoptosis; pollution; tributyltin; multixenobiotic resistance; sponges; geodia cydonium; Caulerpa toxin

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