engleski

Initial monolayer formation in marine biofilms

Studies of biofilm formation are essential for understanding and control of processes in natural aquatic systems and for industrial application. Experiments with expanding mercury electrode/seawater interface offer an approach to studies of initial monolayer in biofilm formation in seawater. Chronoamperometry of dissolved oxygen was used as a measuring technic. Dynamics of film formation is measured as a time which is necessary to reach a full coverage of the electrode by adsorption of dissolved biomolecules and/or adhesion of cells suspended in aqueous electrolyte solution. Phytoplankton organism D.tertiolecta, a free-living bacteria strain and hydrophobic Acinetobacter sp. were chosen as model organisms. Dextran and albumin were chosen as dissolved biomolecules, and triton T-X-100 as nonionic detergent. We have demonstrated direct competition of adsorption of dissolved biomolecules and adhesion of cells in the initial monolayer formation at a freshly exposed surface and a high rate of such processes.

biofilm; D.tertiolecta; initial monolayers; marine bacteria; mercury electrode

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