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Removal of detergent and solvent from solvent-detergent-treated immunoglobulins

The solvent-detergent (S/D) method was applied for inactivation of lipid-enveloped viruses during the production of immunoglobulins. Amberlite XAD-7 resin was used for removal of solvent (tri-n-butyl phosphate, TnBP) and detergent (Triton X-100) after the performed S/D inactivation procedure. The S/D reagents from the immunoglobulin preparation were adsorbed on Amberlite XAD-7, while immunoglobulins passed through the column and retained their biological activity. Using the method developed here, the final immunoglobulin preparation contains less than 1 ppm of Triton X-100 and less than 2 ppm TnBP. (C) 1999 Elsevier Science B.V. All rights reserved. [References: 22]

solvent-detergent method; amberlite xad-7 resin; immunoglobulins; triton x-100; tributyl phosphate

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