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Vitamin C and Glutathione with a Chemical Prepared Tubular Silver Electrode (CROSBI ID 90504)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Kolar, Mitja ; Dobčnik, Danilo ; Radić, Njegomir Vitamin C and Glutathione with a Chemical Prepared Tubular Silver Electrode // Die Pharmazie, 55 (2000), 12; 913-916-x

Podaci o odgovornosti

Kolar, Mitja ; Dobčnik, Danilo ; Radić, Njegomir

engleski

Vitamin C and Glutathione with a Chemical Prepared Tubular Silver Electrode

This paper describes the preparaion and use of a tubular electrode by means of chemical pretreatment of a silver tube with mercuric (II) chloride solution and iodide solution in flow injection analysis (FIA). The electrode was used as a potentiometric sensor for the indirect determination of vitamin C and glutathione in a carrier stream containing iodine. A simple FIA system that consist of a peristaltic pump, an injection T valve, a tubular silver electrode and a saturated calomel reference electrode /SCE) was used. Some typical FIA parameters as flow rate, tube length, sample volume and composition of the carrier stream were varied. After optimisation of these parameters, the electrode was further charcherised by a constant linear response with the concentration range for vitamin C between 5×10-5 and 5×10-3 M at the slope of 60.5+-1.0 mV/p (vitamin C). Glutathione has a linear concentration range between 5×10-5 and 5×10-2 M at a slope of 55.2+-1.0 mV/p (glutathione). Some pharmaceutical products containing vitamin C were also tested. These results can be compared to results obtained by the standard volumetric method for the determination of vitamin C and are also in good agreement with values declared by pharmaceutical manufactures.

flow injection analysis; tubular siver electrode; potentiometric; vitamin C; glutathione

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Podaci o izdanju

55 (12)

2000.

913-916-x

objavljeno

0031-7144

Povezanost rada

Kemija

Indeksiranost