engleski

Expression of multixenobiotic resistance mechanism in a marine mussel Mytilus galloprovincialisas as a biomarker of exposure to polluted environment

The gills of mussel Mytilus galloprovincialis express a multixenobiotic resistance mechanism (MXRM) mediated by P-glycoprotein activity, as evidenced by the rate of (G-H-3)vincristine accumulation and its sensitivity to verapamil, as well as by the direct immunochemical detection of P-glycoprotein by Western blotting analysis. The MXRM is induced at polluted sites: gills from mussels living at polluted sites accumulate less vincristine, the vincristine accumulation is less sensitive to verapamil, and in most cases express higher levels of P-glycoprotein. The state of induction of functional activity of MXRM is proportional to the level of pollution, as demonstrated at sites along the | well defined pollution gradient in the aquatorium of Rovinj, Northern Adriatic. Since the immunological expression of P-glycoprotein may be compromised by high interindividual differences, season, and some as yet unknown factors, it could not be used as a single marker of exposure. However, the rate of vincristine accumulation and its sensitivity to verapamil offer a new, informatively powerful, molecular biomarker of exposure

Mytilus galloprovincialis; multixenobiotic resistance; P-glycoprotein; functional analysis; immunodetection; induction; biomarker

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