engleski

Microbiological analysis of periodontal therapy

Aim of this study was to show the effect of scaling and root planing on clinical and microbiological factors in 28 patients with chronic and aggressive periodontitis. Clinical assessment and microbiological testing were performed prior to, and three months after mechanical therapy. Presence of dental plaque, gingival bleeding, pocket depth and attachment loss were assessed. Samples of subgingival plaque were analysed by polymerase chain reaction method on the presence of seven bacterial pathogens. Clinical and bacterial parameters were analysed by Wilcoxon Rank test. Mean pocket depth significantly decreased from 3.9 to 3.0 mm. Clinical attachment level decreased moderately from 4.1 to 3.8 mm. Mean plaque and gingival bleeding values also decreased. Prevalence of subgingival pathogens in relation to subjects was as follows: one pathogenic species was found in 28.6%, two were found in 46.4% and three in 14.3% subjects. Most prevalent pathogens were B. forsythus in 85.7%, P. gingivalis in 32.1 %, A. actinomycetemcomitans and F. nucleatum in 32.1% subjects. After therapy prevalence of pathogens decreased moderately. Total number of tested pathogens decreased in 12 subjects which was statistically significant (p=0.001). In 16 subjects the number of pathogens was the same. Treatment was only partially effective in decreasing the prevalence of pathogens. This indicates the need for additional antimicrobial therapy in advanced cases of periodontitis.

periodontitis; subgingival plaque; PCR; B. forsythus; P.gingivalis; A. actinomycetemmcomitans; F.nucleatum

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