engleski

4-Chloroindole-3-acetic and indole-3-acetic acids in Pisum sativum

Endogenous IAA and 4-chloroindole-3-acetic acid (4-Cl-IAA) were analyzed in vegetative and reproductive tissues of the garden pea (Pisum sativum L.) using GC-MS selected ion monitoring in the presence of stable-isotope labeled internal standards. In fruit collected 3 - 8 days after anthesis (DAA) conjugates of both auxins were more abundant than the free hormones. Auxin levels (ng/g fr. wt) in the seeds were higher by 1 - 2 orders of magnitude than in the pericarps. However, as seeds are small at this stage, the pericarp nevertheless contains a substantial fraction of the overall quantity of IAA and 4-Cl-IAA. While, in young fruit tissues, IAA was more abundant than 4-Cl-IAA, the opposite was true for seeds at the table-ready stage and for roots of nine-day-old seedlings. Our data suggest that both IAA and 4-Cl-IAA are required to coordinate the vegetative and reproductive growth of pea plants.

Pisum sativum; Fabaceae; garden pea; hormone analysis; free and conjugated auxin; 4-chloroindole-3-acetic acid; IAA

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