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Plasma metabolites and enzymes of bluefin tuna, Thunnus thynnus and liver histology

Virtually no comprehensive work has been published on tuna plasma chemistry parameters and its correlation with tissue histological status. This work will be useful for establishing plasma biochemistry reference values for bluefin tuna. Heparinized blood samples were collected from bluefin tuna. Plasma biochemical analyses were performed using standard techniques. Organ tissues were processed histologically and stained with haematoxylin/eosin. Mean, median, standard deviation, standard error of mean, 25th percentile, 75th percentile and range difference of minimum and maximum values were calculated for each variable measured. Obtained values represent preliminary results. All blood biochemistry parameters, except ALT, passed a Kolmogorov-Smirnov normality test. In a number of hepatocytes, different degrees of fatty changes were visible. Normal ranges of plasma activity vary between species and normal values must be produced from a representative population of each species. This work will be followed up by other studies on blood chemistry values of bluefin in the Adriatic Sea involving more individuals from several locations that could provide a base for reference levels

Thunnus thynnus ; blood biochemistry ; liver histology

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