engleski

Study of Lipase Enantioselectivity

In biotechnology, microbial lipases are used to obtain stereo-clean compounds. Burkholderia cepacia lipase (BCL) catalyses esterification of the aryl secondary alcohol 1-phenoxy-2-hydroxybutane with high enantioselectivity: In our earlier study we determined structural parametres important for stereoselectivity of BCL2-3 towards alcohols. In the present work we investigate different binding modes of the slow and the fast reacting secondary alcohol enantiomers in more detail and elucidate possible reaction pathways.

enzymes

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