Serotonin and immune response: effect of the amine on in vitro proliferation of rat lymphocytes (CROSBI ID 481614)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Štefulj, Jasminka ; Čičin-Šain, Lipa ; Schauenstein, Konrad ; Jernej, Branimir
engleski
Serotonin and immune response: effect of the amine on in vitro proliferation of rat lymphocytes
The effect of serotonin (5-hydroxytryptamine, 5HT) on the in vitro proliferation of mitogen-stimulated lymphocytes was studied in primary cultures of rat spleen cells. 5HT was added to the cultures 1 h prior to the mitogen, at final concentrations from 10-13 up to 10-2 M. T and B cell mitogens (Concanavalin A, pokeweed mitogen and lipopolysaccharide) were used at suboptimal and optimal concentrations. The cell proliferation was measured 24-72 h after the addition of mitogen. The effect of each 5HT concentration was studied on a group of 6-12 animals and was expressed as a percentage of the control values obtained with mitogen alone. No significant effect of 5HT at concentrations from 10-13 to 10-5 M was found. At concentrations of >10-4 M, a regular dose-dependent inhibition of the lymphocyte proliferation appeared, the concentration producing the half-maximal effect (IC 50) being 6x10-4 M. The observed suppression was not due to 5HT cytotoxicity toward spleen cells. With the experimental system used, we failed to confirm an immunostimulatory effect of 5HT in the range of concentrations of its receptor sensitivities or lower, but found a clear-cut immunoinhibitory effect at higher concentrations.
serotonin; lymphocyte; proliferation
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Podaci o prilogu
100-100.
2001.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
Croatian Congress of pharmacology with international participation (3 ; 2001)
poster
18.09.2001-21.09.2001
Zagreb, Hrvatska
Povezanost rada
Temeljne medicinske znanosti