Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma (CROSBI ID 265102)

Prilog u časopisu | izvorni znanstveni rad

Trbojević-Akmačić, Irena ; Nemec, Blaž ; Vidic, Urška ; Malić, Suzana ; Miklić, Karmela ; Černigoj, Urh ; Vidič, Jana ; Lendero Krajnc, Nika ; Štrancar, Aleš ; Lauc, Gordan et al. Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma // Croatica chemica acta, 89 (2016), 2; 203-211. doi: 10.5562/cca2815

Podaci o odgovornosti

Trbojević-Akmačić, Irena ; Nemec, Blaž ; Vidic, Urška ; Malić, Suzana ; Miklić, Karmela ; Černigoj, Urh ; Vidič, Jana ; Lendero Krajnc, Nika ; Štrancar, Aleš ; Lauc, Gordan ; Lenac Roviš, Tihana ; Pučić-Baković, Maja

engleski

Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma

Changes in protein glycosylation are related to different diseases and have a potential as diagnostic and prognostic disease biomarkers. Transferrin (Tf) glycosylation changes are common marker for congenital disorders of glycosylation. However, biological interindividual variability of Tf N-glycosylation and genes involved in glycosylation regulation are not known. Therefore, high-throughput Tf isolation method and large scale glycosylation studies are needed in order to address these questions. Due to their unique chromatographic properties, the use of chromatographic monoliths enables very fast analysis cycle, thus significantly increasing sample preparation throughput. Here, we are describing characterization of novel immunoaffinity-based monolithic columns in a 96- well plate format for specific high-throughput purification of human Tf from blood plasma. We optimized the isolation and glycan preparation procedure for subsequent ultra performance liquid chromatography (UPLC) analysis of Tf N- glycosylation and managed to increase the sensitivity for approximately three times compared to initial experimental conditions, with very good reproducibility.

high-throughput ; immunoaffinity chromatography ; monoliths ; N-glycosylation ; oriented antibody immobilization ; transferrin

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

89 (2)

2016.

203-211

objavljeno

0011-1643

10.5562/cca2815

Povezanost rada

Biologija, Biotehnologija, Kemija

Poveznice
Indeksiranost