Fc-linked immunoglobulin G N-glycosylation in Fcy receptor knock-out mice (CROSBI ID 679729)
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Podaci o odgovornosti
Zaytseva, Olga O. ; Seeling, Michaela ; Krištić, Jasminka ; Lauc, Gordan ; Pezer, Marija ; Nimmerjahn, Falk
engleski
Fc-linked immunoglobulin G N-glycosylation in Fcy receptor knock-out mice
Immunoglobulin G (IgG) is involved in a number of both protective and pathogenic immune response pathways. By binding to receptors specific for its Fc region – Fcγ receptors (FcγRs) – expressed on the surface of various immune cells, IgG is involved in the regulation of both, the innate and adaptive arms of the immune response. In mice, as well as in humans, each IgG molecule contains a single biantennary N-glycan covalently attached to the conserved N-glycosylation site on each of its heavy chains. Multiple in vitro studies have confirmed that the composition of IgG N-glycans affects its binding to the FcγRs. Polyclonal IgG glycosylation is known to vary markedly in different physiological and pathological states. However, little is known about the mechanisms that regulate the composition of IgG glycome. In this study we investigated whether a feedback regulation of IgG glycosylation depending on the available repertoire of FcRs exists in vivo. To this purpose, we analyzed serum IgG glycosylation in mice deficient for a single FcγR (FcγRI-/-, FcγRIIB-/-, FcγRIII-/-, FcγRIV-/-), all activating FcγRs (FcRγ-/-) or all FcγRs (FcRγ-/-FcγRIIB-/-) on C57Bl/6 background. We also analyzed IgG N-glycan profiles of FcγRIIB-/-, FcRγ-/- and FcRγ-/- FcγRIIB-/- on Balb/c background. IgG isolated from serum was digested with trypsin and the resulting Fc-glycopeptides were analysed with liquid chromatography - electrospray ionization - mass spectrometry.
Fcγ receptor ; IgG N-glycan profile ; immunoglobulin G ; N-glycosylation
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Podaci o prilogu
xx
2018.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
EMBO Workshop: Antibodies and Complement: Effector Functions, Therapies and Technologies
poster
28.06.2018-01.07.2018
Girona, Španjolska