N‐Glycan Analysis by Ultra‐Performance Liquid Chromatography and Capillary Gel Electrophoresis with Fluorescent Labeling (CROSBI ID 273146)
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Podaci o odgovornosti
Hanić, Maja ; Lauc, Gordan ; Trbojević‐Akmačić, Irena
engleski
N‐Glycan Analysis by Ultra‐Performance Liquid Chromatography and Capillary Gel Electrophoresis with Fluorescent Labeling
Glycans are a class of macromolecules essential for all forms of life. They embellish various proteins and other macromolecules in organisms and are responsible for their proper functioning. Because their complex structure is determined by genetic and environmental factors, analysis of such molecules is rather demanding. Liquid chromatography (high- performance and ultra-performance, HPLC and UPLC, respectively) analysis has been used for the purpose of glycoprofiling for years and it is a well-established method regarding its robustness, reproducibility, and high throughput. Another orthogonal method that is now used in glycoprofiling is capillary gel electrophoresis (CGE) because it offers powerful separation and distinct sensitivity. The purpose of the following protocols is to present all steps required for release and fluorescent labeling of total N-glycans from blood plasma/serum or isolated glycoprotein (for example, IgG) and their subsequent UPLC or CGE analysis.
N-glycan ; capillary gel electrophoresis ; fluorescent labeling ; liquid chromatography ; posttranslational modification
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Podaci o izdanju
97 (1)
2019.
e95
21
objavljeno
1934-3655
1934-3663
10.1002/cpps.95