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izvor podataka: crosbi

Hydrocortisone and dexamethasone affect galectin-3 expression (CROSBI ID 118348)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Dabelić, Sanja ; Flögel, Mirna ; Dumić, Jerka Hydrocortisone and dexamethasone affect galectin-3 expression // Periodicum biologorum, 107 (2005), 2; 175-181-x

Podaci o odgovornosti

Dabelić, Sanja ; Flögel, Mirna ; Dumić, Jerka

engleski

Hydrocortisone and dexamethasone affect galectin-3 expression

Background and Purpose: Galectin-3, a β -galactoside binding lectin, acts as a strong pro-inflammatory signal. Many immunomodulatory drugs affect signaling pathways that comprise transcription factors involved in regulation of galectin-3 gene (LGALS3) expression. The aim of our study was to investigate the effects of steroidal anti-inflammatory drugs (hydrocortisone - HC and dexamethasone - Dex) on the expression of galectin-3, both on the mRNA and the protein level. Materials and Methods: The human monocytic cell line THP-1 was exposed to various concentrations of HC (0.1, 1, 5 and 10 μ M) and Dex (0.1, 1, 10 and 100 nM) during 1, 3, 5, 24, 48 or 72 hrs. Relative RT-PCR method and GeneScan analysis software were used for assessing galectin-3 mRNA level and chemiluminescent-western blot analysis for measuring galectin-3 level. Results: The results showed that both drugs in all applied concentrations halved the galectin-3 mRNA level already after 1 h of exposure. The decrease continued and after 72 hours mRNA level dropped on 3.8-15.4 % of the initial value. The protein expression of galectin-3 didn’ t follow the same pattern ; although both drugs in all applied concentration provoked the reduction of galectin-3 level, the decrease was observed not until 48 and 72 hours of exposure, when galectin-3 level fell on 71 % to 38 % of the initial value, respectively. Conclusion: Steroidal anti-inflammatory drugs cause the reduction of both gene and protein expression of galectin-3, but the intensity of the changes and the time of their appearance strongly depends on the kind and the concentration of the drugs.

hydrocortisone; galectin-3; dexamethasone; LGALS3

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Podaci o izdanju

107 (2)

2005.

175-181-x

objavljeno

0031-5362

Povezanost rada

Biologija

Indeksiranost