engleski

LPS-induced changes of galectin-3 expression are modulated by indomethacin but not aspirin

Galectin-3 is a multifuntional β -galactoside binding lectin which in general acts as a strong pro-inflammatory signal that modulates cell proliferation and adhesion, chemotaxis, phagocytosis and synthesis of inflammatory mediators. We have previously shown that different immunomodulatory drugs affect LGALS3 gene and galectin-3 expression in monocytic THP-1 cell line, and that their effects depend on drug type and applied concentration as well as time of the exposure. Monocytes are exquisitely sensitive to lipopolysaccharides (LPS) and respond by expressing many inflammatory cytokines. Therefore, the aim of our study was to investigate the effects of non-steroidal immunomodulatory drugs (aspirin and indomethacin), applied in therapeutic ranges on the expression of LGALS3 and galectin-3 in monocytic THP-1 cells activated by LPS. The targeted mRNA level was evaluated using relative RT-PCR technique and analysis on the AbiPrism 310 Genetic Analyser. Galectin-3 expression in cell homogenates was determined by western-immunoblot analysis. The results showed that LPS induced LGALS3 and galectin-3 expression, and that the effects depend on the concentration of LPS and time of the exposure. The LPS-induced increase of LGALS3 and galectin-3 expression was not affected by the addition of aspirin, but it was, on the other hand, additionally enhanced by indomethacin. The effects of indomethacin on the galectin-3 protein level were delayed comparing to the effects on mRNA level. These findings represent important step in the understanding of the immunomodulatory activities of non-steroidal anti-inflammatory drugs, as well as the role of galectin-3 in the physiology of monocytes/macrophages.

galectin-3; indomethacin; aspirin

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