engleski

Changes of serum glycans in acute inflammation

Although acute inflammatory response is generally a unique homeostatic mechanism, there are differences associated with the nature and site of inflammation. We examined changes of N-linked glycans released from serum of a patient with sepsis and a patient with acute pancreatitis during first eight days of disease and compared them to the control sample. Sera were taken from patients at time of reporting to hospital and then three more times. The blood from healthy individual was drawn on one occasion only. Glycans were released using N-glycosydase F and subjected to normal phase HPLC combined with exoglycosidase digestions and mass spectrometry. Levels of identified structures have been followed through the course of disease and compared to the control. Changes of serum glycans were found to occur very early in acute inflammation. Changes that are presumably part of regulatory processes during inflammation have been observed in tri- (A3G3S3 and A3G3S3F) and tetra-sialylated structures, mannose structures, level of fucosylation (both core and outer arm), and the degree of branching. The proportions of different glycans were changing daily and some differences were also observed between sepsis and pancreatitis, presumably as a reflection of the fact that in these two conditions the acute phase response is triggered by a different stimulus and is associated with different patterns of production of specific cytokines.

glycans; acute inflammation; sepsis; pancreatitis

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