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Fusogenic activity of PEGylated pH-sensitive liposomes (CROSBI ID 202233)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Vanić, Željka ; Barnert, Sabine ; Süss, Regine ; Schubert, Rolf Fusogenic activity of PEGylated pH-sensitive liposomes // Journal of liposome research, 22 (2012), 2; 148-157. doi: 10.3109/08982104.2011.633267

Podaci o odgovornosti

Vanić, Željka ; Barnert, Sabine ; Süss, Regine ; Schubert, Rolf

engleski

Fusogenic activity of PEGylated pH-sensitive liposomes

The aim of this study was to investigate the fusogenic properties of poly(ethylene glycol) (PEG)ylated dioleoylphosphatidylethanolamine/cholesteryl hemisuccinate (DOPE/CHEMS) liposomes. These pH-sensitive liposomes were prepared by incorporating two different PEG lipids: Distearoylphosphatidylethanolamine (DSPE)-PEG2000 was mixed with the liposomal lipids using the conventional method, whereas sterol-PEG1100 was inserted into the outer monolayer of preformed vesicles. Both types of PEGylated liposomes were characterized and compared for their entrapment efficiency, zeta potential and size, and were tested in vitro for pH sensitivity by means of proton-induced leakage and membrane fusion activity. To mimic the routes of intracellular delivery, fusion between pH-sensitive liposomes and liposomes designed to simulate the endosomal membrane was studied. Our investigations confirmed that DOPE/CHEMS liposomes were capable of rapidly releasing calcein and of fusing upon acidification. However, after incorporation of DSPE-PEG2000 or sterol-PEG1100 into the membrane, pH sensitivity was significantly reduced ; as the mol ratio of PEG-lipid was increased, the ability to fuse was decreased. Comparison between two different PEGylated pH-sensitive liposomes showed that only vesicles containing 0.6 mol% sterol-PEG1100 in the outer monolayer were still capable of fusing with the endosome-like liposomes and showing leakage of calcein at pH 5.5.

DSPE-PEG2000; Fusion; PH-sensitive liposomes; Sterol-PEG1100

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Podaci o izdanju

22 (2)

2012.

148-157

objavljeno

0898-2104

10.3109/08982104.2011.633267

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