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Effect of 16S rRNA methyltransferase RmtD on biofilm formation and pyocyanin production in Pseudomonas aeruginosa PAO1

Pseudomonas aeruginosa is one of the leading causes of life-threatening nosocomial infections. The problem of antibiotic resistance in P. aeruginosa is highlighted by the emergence of multi-drug resistant strains. One of the pathogenic potential of P. aeruginosa is based on formation of biofilm during chronic infections. The regulation of many virulence associated factors, such as pyocyanin production and as well biofilm formation in P. aeruginosa, are under the control of quorum sensing system. RmtD is a methyltransferase that confers resistance to aminoglycosides, andwas identified in an P. aeruginosa clinical isolate. In this study, we investigated the link between 16S rRNA methyltransferase RmtD and biofilm formation and production of virulence factors in P. aeruginosa. Expression of RmtD in P. aeruginosa PAO1, demonstrated that RmtD positively affected biofilm formation in P. aeruginosa PAO1. Furthermore, linking its role in regulating virulence factors production such as pyocyanin, revealed that RmtD negatively affected the production of this virulent factor, regulated by quorum sensing system. Our further interest was to investigate the effect of RmtD on biofilm formation in P. aeruginosa mutants defective in production of RhlI/R quorum sensing system. We observed positive effect of RmtD on biofilm formation in these mutant strains. These results indicate that 16S rRNA methyltransferase RmtDexerts both positive and negative effect on quorum sensing system in P. aeruginosa PAO1 and further studies will focus on deciphering the link between 16S rRNA methyltransferase RmtD and quorum sensing.

Pseudomonas aeruginosa PAO1; 16S rRNA methyltransferase RmtD; biofilm; pyocyanin

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