Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Plasma N-glycome composition associates with chronic low back pain (CROSBI ID 259557)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Trbojević-Akmačić, Irena ; Vučković, Frano ; Vilaj, Marija ; Skelin, Andrea ; Karssen, Lennart C. ; Krištić, Jasminka ; Jurić, Julija ; Momčilović, Ana ; Šimunović, Jelena ; Mangino, Massimo et al. Plasma N-glycome composition associates with chronic low back pain // Biochimica et biophysica acta. G, General subjects, 1862 (2018), 10; 2124-2133. doi: 10.1016/j.bbagen.2018.07.003

Podaci o odgovornosti

Trbojević-Akmačić, Irena ; Vučković, Frano ; Vilaj, Marija ; Skelin, Andrea ; Karssen, Lennart C. ; Krištić, Jasminka ; Jurić, Julija ; Momčilović, Ana ; Šimunović, Jelena ; Mangino, Massimo ; De Gregori, Manuela ; Marchesini, Maurizio ; Dagostino, Concetta ; Štambuk, Jerko ; Novokmet, Mislav ; Rauck, Richard ; Aulchenko, Yurii S. ; Primorac, Dragan ; Kapural, Leonardo ; Buyse, Klaas ; Mesotten, Dieter ; Williams, Frances M.K. ; van Zundert, Jan ; Allegri, Massimo ; Lauc, Gordan

engleski

Plasma N-glycome composition associates with chronic low back pain

BACKGROUND: Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging. Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors. LBP may be characterized by an underlying inflammatory process. Previous studies indicated a connection between inflammatory response and total plasma N- glycosylation. We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease. METHODS: Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra- performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up. Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery. RESULTS: We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls. Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP. CONCLUSIONS: Observed changes in CLBP on the plasma N-glycome level are consistent with N- glycosylation changes usually seen in chronic inflammation. GENERAL SIGNIFICANCE: To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.

Glycan biomarker ; Low back pain ; Plasma N-glycosylation ; Retrospective study

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

1862 (10)

2018.

2124-2133

objavljeno

0304-4165

10.1016/j.bbagen.2018.07.003

Povezanost rada

Temeljne medicinske znanosti

Poveznice
Indeksiranost