engleski

Paraoxonase 1 activity in patients with premalignant lesion of the cervics

Paraoxonase 1 (PON1) is a enzyme whic is associated with high density lipoprotein (HDL) in serum. PON1 posses paraoxonase, arylesterase and lactonase activity and hidrolyze different kindes of supstrate. Also, PON1 have antioxidant/antiatherogenic activity, and it removes carcinogenic radicals of lipid peroxidation in human body. It was established earlyer that patients with premalignant lesions of cervix have increased lipid peroxidation and changes in antioxidative system, which points importance of oxidative stress in early phase of carcinogenesis. AIM: We carried out this study in order to determine PON1 arylesterase activity in patients with premalignant lesions of the cervix. METHODS: Study included 65 women [38 (32-46) years] with a biopsy confirmed diagnosis of cervical intraepitelial neoplasia (CIN), 18 patients had CIN1, 23 had CIN2 and 24 had CIN3. 109 healty volonteers [37 (29-42) years] without any cervical or other disease were enroled in the study as a control group. Concentration of HDL cholesterole and apolipoprotein AI (apoAI) were determined by commercially available reagents. PON1 arylesterase activity was assayed spectrophotometrically with phenilacetate as a substrate. We standardized PON1 arylesterase activity according to the concentration of HDL- cholesterol RESULTS: PON1 arylesterase activity was significantly reduced in group of patients (53 ± 20 kU/L vs 75 ± 18 kU/L ; P<0.001 ; Figure 1.). PON1 arylesterase activity standardized on the concentration of HDL (36 ± 28 kU/mmol vs 41 ± 35 kU/mmol ; P=0.004 ; Figure 2.) and on the concentration of apoAI [28 (23 – 35) kU/g vs 42 (35 – 51) kU/g ; P<0.001 ; Figure 3.) were lower in the group of patients with premalignant lesion of cervix compared to the healthy volonters. Diagnostic accuracy of PON1 arylesterase activity in discriminating between healthy subjects and patients with premalignant lesion of the cervix was good [AUC (95% CI): 0.784 80.715 – 0.842). Sensitivity and specificity with corresponding 95% confidence intervals (95% CI) was 65 (52 – 76) and 85 (77 – 91) respectively. Cut off value was <=56 kU/L. CONCLUSION: Our results suggest that PON1 arylesterase activity is reduced in patients with premalignant lesions of the cervix. Furthermore results of diagnostic accuracy in discriminating between healthy and individuals with premalignant lesions of the cervix indicated that PON1 arylesterase activity could be considered as a potentially valuable biomarker for with premalignant lesions of the cervix.

Paraoxonase , premalignant lesion of the cervics

nije evidentirano