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Comparative Analysis and Validation of Different Steps in Glycomics Studies (CROSBI ID 64065)

Prilog u knjizi | izvorni znanstveni rad | međunarodna recenzija

Trbojević-Akmačić, Irena ; Ugrina, Ivo ; Lauc, Gordan Comparative Analysis and Validation of Different Steps in Glycomics Studies // Proteomics in Biology, Part B / Shukla, Arun K. (ur.). Cambridge (MA): Elsevier, 2017. str. 37-55 doi: 10.1016/bs.mie.2016.09.027

Podaci o odgovornosti

Trbojević-Akmačić, Irena ; Ugrina, Ivo ; Lauc, Gordan

engleski

Comparative Analysis and Validation of Different Steps in Glycomics Studies

Large-scale glycomics studies enable identification of aberrant glycosylation patterns in disease and provide information about functional relevance of individual glycans through genome-wide association studies. Developed high-throughput methodologies have to be sensitive, robust, and stable during long periods of time (few months) to be able to reliably detect small biological variations in glycosylation. Here, we describe a simple, robust, and affordable protocol for immunoglobulin G N-glycan analysis by hydrophilic interaction liquid chromatography–ultra-performance liquid chromatography (HILIC-UPLC), as well as useful strategies for method optimization: Plackett–Burman screening design and analysis of source of variation. We put our focus on experimental design for high-throughput glycan analysis, critical steps in sample preparation procedure for obtaining high-quality data, and propose a validation protocol relevant for high-throughput methods in terms of their long-term robustness and ability to detect biologically relevant changes in glycosylation. The quality of the procedure was assessed by employing appropriate experimental designs and subsequent statistical techniques.

Glycans ; High throughput ; Glycome analysis ; HILIC ; UPLC ; Method validation

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Podaci o prilogu

37-55.

objavljeno

10.1016/bs.mie.2016.09.027

Podaci o knjizi

Proteomics in Biology, Part B

Shukla, Arun K.

Cambridge (MA): Elsevier

2017.

978-0-12-809743-4

0076-6879

Povezanost rada

Biologija, Kemija

Poveznice
Indeksiranost