engleski

Antiproliferative and Antioxidant Activities of the Organic Extract and Semi-purified Fractions from Gorgonian Coral Eunicella cavolini

The yellow gorgonian, Eunicella cavolini (Koch, 1887), is a common and widespread gorgonian species in the Mediterranean Sea known as an abundant source of structurally different primary and secondary metabolites [1]. In a large number of studies on gorgonians, diterpenes, sesquiterpenes, alkaloids and steroids, often unique in carbon skeleton and stereochemistry were isolated and characterized. Many of these compounds have shown a wide range of biological activities, which has attracted the attention of the scientific community, particularly in the search for new anticancer compounds of natural origin [2–4]. Within the platform of Adriatic Sea bioprospecting, we evaluated the antiproliferative and antioxidant activities of the organic extract and corresponding, four semi-purified fractions from E. cavolini. The organic extract was obtained by using dichloromethane/methanol solvent system (3:1) followed by fractionation using a solvent system (cyclohexane, ethyl acetate, methanol) of different ratios. The antiproliferative activity was assayed against four human cancer cell lines (SW620, CFPAC-1, MCF-7 and HeLa) as well as non- transformed fibroblast cell line (HFF-1) using MTT [3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] colorimetric method. The antioxidant activity was examined by 1, 1- diphenyl-2-picrylhydrazyl (DPPH) assay. All tested samples displayed strong antiproliferative activity with IC50 value around or less than 50 μg/mL, except for the first fraction, F1 (c-hexane/ethyl acetate, 75:25) against SW620 colon cancer cells. Fraction, F3 (ethyl acetate) exhibited the strongest radical scavenging activity with IC50 value of 0.76 mg/mL. Further studies will include the isolation of pure bioactive secondary metabolites and the assignation of their structures by NMR spectroscopy. ACKNOWLEDGMENTS AND FUNDING We would like to thank Croatian Government and the European Union (European Regional Development Fund—the Competitiveness and Cohesion Operational Programme - KK.01.1.1.01) for funding this research through project Bioprospecting of the Adriatic Sea (KK.01.1.1.01.0002) granted to The Scientific Centre of Excellence for Marine Bioprospecting - BioProCro. We also acknowledge the project “Research Infrastructure for Campus-based Laboratories at University of Rijeka”, co- financed by European Regional Development Fund (ERDF) and the University of Rijeka research grant uniri-biomed-18-133 (1277). We would also like to thank Croatian Science Foundation project ‘Career Development of Young Researchers–Training of New PhDs. REFERENCES [1] Samorì, C. ; Costantini, F. ; Galletti, P. ; Tagliavini, E. ; Abbiati, M. Inter- and Intraspecific Variability of Nitrogenated Compounds in Gorgonian Corals via Application of a Fast One-Step Analytical Protocol. Chem. Biodivers. 2018, 15(1), e1700449. [2] Ioannou, E. ; Abdel-Razik, A.F. ; Alexi, X. ; Vagias, C. ; Alexis, M.N. ; Roussis, V. 9, 11- Secosterols with Antiproliferative Activity from the Gorgonian Eunicella Cavolini. Bioorg. Med. Chem. 2009, 17(13), 4537–4541. [3] Ioannou, E. ; Abdel-Razik, A.F. ; Alexi, X. ; Vagias, C. ; Alexis, M.N. ; Roussis, V. Pregnanes with Antiproliferative Activity from the Gorgonian Eunicella Cavolini. Tetrahedron. 2008, 64(51), 11797–11801. [4] Deghrigue, M. ; Dellai, A. ; Akremi, N. ; Le Morvan, V. ; Robert, J. ; Bouraoui, A. Evaluation of Antiproliferative and Antioxidant Activities of the Organic Extract and Its Polar Fractions from the Mediterranean Gorgonian Eunicella Singularis. Environ. Toxicol. Pharmacol. 2013, 36(2), 339–346.

Eunicella cavolini extract/fractions ; antiproliferative activity ; antioxidant activity

nije evidentirano