Diversity of marine Cyanobacteria in coastal waters and sediments in central and southern eastern Adriatic sea (CROSBI ID 680053)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Kolda, Anamarija ; Ljubešić, Zrinka ; Mucko, Maja ; Žunić, Jakov ; Gavrilović, Ana ; Jug-Dujaković, Jurica ; Pikelj, Kristina ; Šoštarić Vulić, Zvjezdana ; Kapetanović, Damir
engleski
Diversity of marine Cyanobacteria in coastal waters and sediments in central and southern eastern Adriatic sea
Mediterranean Sea, characterized by extreme oligotrophy and bathymetrically divided into northern, central and the southern Adriatic basin. The aims of the study were to determine Cyanobacteria diversity within the microbial community in the water and sediment, identify the environmental factors in coastal waters of the sites and connect these findings to get insight into marine cyanobacterial ecology. Sampling was conducted in all four seasons during 2017 at two sampling points in coastal eastern Adriatic Sea. First sampling point in central Adriatic is oligotrophic, in a clove under the influences of the open sea, and second in southern Adriatic is moderately eutrophic, in the bay influenced by freshwater riverin input. Water samples were taken with Niskin sampler throughout the water column (maximum depth 20 m), while sediment samples were taken by the diver. Physico-chemical properties of the seawater were measured in situ (Secchi depth, turbidity, conductivity, temperature, salinity, oxygen concentrations and saturations, pH, TDS and ORP) and concentrations of N, P, Si and SiO2 were measured in the laboratory. Total bacterial DNA was extracted from water and sediment and the relative abundance and composition of 16S rRNA phylogenetic marker was determined using 16S rRNA (V1–V3 region) Illumina MiSeq. Bioinformatics and statistical analysis were performed using QIIME2 and RStudio. Water samples in both sampling sites were dominated by Synechococcus and Prochlorococcus, with many reads determined as “Other” and/or assigned as cyanobacterial chloroplast 16S rRNA. These reads had to be filtered after the assign taxonomy step, checked in NCBI database and a phylogenetic tree was constructed to get more precise taxonomical determination. The same procedure was conducted for sediment samples, which harboured great abundances of chloroplast reads, but also Pleurocapsa and uncultured taxa at both sampling sites, while Lyngbya and Oscillatoria were detected only at the second location.
Cyanobacteria ; Adriatic sea ; water column ; sediment ; NGS
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Podaci o prilogu
190-190.
2019.
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objavljeno
10.1080/09670262.2019.1626628
Podaci o matičnoj publikaciji
European journal of phycology
Ljubešić, Zrinka ; Bosak, Sunčica
European Journal of Phycology
0967-0262
1469-4433
Podaci o skupu
7th European Phycological Congress
poster
25.09.2019-30.09.2019
Zagreb, Hrvatska
Povezanost rada
Biologija, Interdisciplinarne prirodne znanosti