hrvatski

Cito/genoprotektivni i antioksidacijski učinci meda obične planike na oštećenja ljudskih limfocita izazvana UVB zračenjem u uvjetima in vitro/Assessment of cyto/genoprotective and antioxidative properties of strawberry tree honey against UVB radiation in vitro

Present in vitro study investigated protective role of strong antioxidant strawberry tree honey and its main bioactive constituent homogentisic acid (HGA) against the cyto/genotoxic effects induced by UVB radiation in human peripheral blood lymphocytes. Tested concentrations corresponded to the daily intake of honey with its matched content of HGA, as well as ten-fold higher concentrations of both agents. Delivered UVB dose was 2 kJ/m2 and corresponded to medial erythema dose. We evaluated differences between two experimental approaches: one set of lymphocyte cultures/blood was pre-treated for two hours before the UVB exposure started, while the other set was treated for two hours after UVB exposure. Lymphocyte viability was checked using the apoptosis/necrosis assay. Genotoxicity was studied by the alkaline comet assay. Lipid peroxidation in plasma samples was measured using thiobarbituric acid reactive substance (TBARS) assay. We found differences in viability between lymphocyte cultures pre-treated with tested compounds prior to UVB exposure and those that were treated. After treatment, honey offered better cytoprotection than HGA, possibly due to its complex phytochemical profile. However, HGA showed better genoprotective properties. The levels of primary DNA damage also differed between the tested cultures. They could be associated with the induction of oxidative stress, as documented by levels of TBARS measured in plasma. Our results suggest that both tested agents effectively ameliorated detrimental effects of UVB radiation. Cyto/genoprotective properties of strawberry tree honey and HGA possible were mediated via mutual interactions of various mechanisms. Among them, most important are scavenging of free radicals and influence on the cellular enzymes involved in the antioxidative defence, as well on the glutathione levels, but this has to be further studied on other in vitro and in vivo model systems.

med obične planike ; homogentizinska kiselina ; UVB ; alkalni komet test ; lipidna peroksidacija

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