engleski

SSR genotpying of endemic Croatian dwarf species Iris adriatica Trinajstić ex Mitić (Iridaceae)

Iris adriatica Trinajstić ex Mitić is a strictly endemic rhizomatous dwarf plant from the I. pumila complex first described in 2002. As an endemic species it is confined to a few Croatian localities in the wider area of Dalmatia (recorded 1980) and the island of Cres (recorded 2014) in the Northern Adriatic. Iris adriatica is classified as a NT (near threatened) species in the IUCN Croatian Red Book of Vascular plants. Recent metabolic profiling of the species revealed a great pharmacological and chemotaxonomic potential with notable diversity between the ecotypes. Despite potential medical applications, the Iris genus has garnered little to no attention from the scientific community as it has mainly been exploited for horticultural purposes. Many of the species, including I. adriatica, have only been taxonomically classified using traditional taxonomical methods of observation and comparison. In the present study we aim to describe the EST-SSR microsatellite profile of the collected I. adriatica samples utilizing eight Iris specific markers. The collected samples cover the known distribution areas and help explore the genetic relationship between the Dalmatia and Cres ecotype localities. Additionally, we aim to expand the available Iris SSR profiles by also characterizing Iris lutescens and Iris pumila. The objective of the study was to provide an insight into the genetic diversity of endemic Iris adriatica along the Eastern coast of the Adriatic.

ecotypes, EST-SSR, genetic diversity, microsatellites, UPGMA dendrogram

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