engleski

Deregulation of monocyte related cytokine/chemokine profile in serum milieu during Puumala virus infection

Changes in circulating cytokine and chemokine levels have been associated with many human diseases, and thus understanding the relationships between these changes and disease is an important. Hemorrhagic fever with renal syndrome (HFRS) is a viral disease caused by hantaviruses, however, it is primarily considered as an immune-mediated disease. Several studies have shown deregulated pro- and anti-inflammatory cytokine/chemokine production during the acute phase of hantavirus infection in patients’ sera. Monocytes/macrophages are important producers of different cytokines/chemokines significantly contributing to the status of the serum milieu. They are also considered as one of the target cells for hantaviruses. The aim of our study was to analyze kinetics of the monocytes/macrophages related cytokines/chemokines in HFRS patients’ serum during the course of disease and correlate the serum profile with the severity of the clinical outcome of Puumala virus (PUUV)-induced disease. Serum samples were collected two times during the disease course. The mean levels of 16 cytokines and chemokines were determined by multiplex immunoassay with magnetic beads. When compared to the controls, some pro-inflammatory cytokines were unaltered (IL-1β, IL-12(p70), IL-23) due to infection, whereas levels of CCL5 and TGF-β1 were significantly lower in infected cases. Significantly elevated levels of IL-15, IL-1RA, IL-18, MIF, CCL2 and CCL4 were detected in PUUV-infected individuals. We found no significant differences in the levels of cytokines/chemokines between the group of patients with moderate symptoms compared to individuals with mild symptoms but cytokines like CCL5 and TGF-β1 showed strong positive correlations with some HFRS-associated pathological laboratory findings

monocytes, macrophages, multiplex immunoassay

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