Mechanism of murine cytomegalovirus entry into the cell nucleus (CROSBI ID 487528)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Kučić, Natalia ; Mahmutefendić, Hana ; Lučin, Pero
engleski
Mechanism of murine cytomegalovirus entry into the cell nucleus
Problem Immediately after the murine cytomegalovirus (MCMV) infection of fibroblasts the phpsphpprotein pp89 (IE), which is the product of immediate-early phase of viral replication is expressed in nucleus. The protein of the early phase of viral replication (E1) is expressed afterwards. Both viral proteins could be detected in cell nucleus 2-3 hrs post infection and be used as indicators of entry of MCMV components into this compartment. Methods of Study In order to investigate the mechanism of the MCMV transport through the cell and viral DNA transport into the cell nucleus the inhibitors of endocytosis (both clathrine and caveolar), vesicular transport and cytoskeletal function were used. The IE and E1 proteins were detected by immunofluorescence and Western blott analysis. Results The inhibitors of endocytosis and vesicular transport as well as depolimerizators of microtubules did not completely inhibit the viral entry into the cell nucleus. The entire block was achieved using the H7 [1-(4-Isoquinolinesulfonyl)-2-methyl-piperazine dihidrochloride], an inhibitor of protein kinase C (PKC) activation. Conclusions These results indicate that the mechanisms of clathrine and caveolar endocytosis are not used for viral entry into the cell. Othervise, for the transport through the cell and for the entry into the nucleus the processes of cytoskeletal phosphorylation are required. Supported by grant No 062006 from the Ministry of Science and Technology, Republic of Croatia.
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Podaci o prilogu
60-x.
2001.
objavljeno
Podaci o matičnoj publikaciji
American Journal of Reproductive Immunology
Gleicher, Norbert
Kopenhagen: Munksgaard
Podaci o skupu
VIII International Congress of Reproductive Immunology.
poster
02.07.2001-06.07.2001
Opatija, Hrvatska