Endocytosis of full and empty Ld molecules in nonpolarized cells (CROSBI ID 487531)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Mahmutefendić, Hana ; Kučić, Natalia ; Lučin, Pero
engleski
Endocytosis of full and empty Ld molecules in nonpolarized cells
Problem To characterize the endocytic pathway of MHC class I molecules, we have studied the expression of Ld molecules on nonpolarized murine P815 cells. These molecules reach the cell surface in the full conformation, composed of properly folded heavy chain, beta2 -microglobulin (beta2m) and peptide, and the empty, which is represented either by non-conformed heavy chains or hetrodimer of weakly associated non-conformed heavy chain and beta2m. Methods of Study In order to investigate the mechanism a palette of inhibitors of the endocytosis and vesicular transport was used. Cycloheximide, an inhibitor of protein synthesis, induced spontaneous endocytosis of Ld molecules and their disappearance from the cell surface or degradation was followed by flow cytometry and Western blott. Results As inhibitors of clathrine endocytosis, chlorpromazine (CP) and peroxovanadate, did not influence the internalization of full and empty Ld molecules. Filipin and nystatin, inhibitors of the caveolar endocytosis, prevented downregulation of empty, but not of full Ld molecules. NH4Cl, prevented the degradation of a majority of empty Ld. As inhibitors of the recycling pathway, CP and bafilomycin A1 caused surface decrease, but intracellular increase of full Ld. Conclusions From these results we can conclude that both full and empty Ld molecules are internalized by caveolar endocytosis. A majority of full Ld molecules are recycled back to the cell surface, and only small amount is degraded. In contrast, one part of the empty Ld is recycled directly to the cell surface, and probably, a major part is transformed in early endosomes into full Ld molecules and transported back to the cell surface. Supported by grant No. 062006 from the Ministry of Science and Technology, Republic of Croatia.
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Podaci o prilogu
60-61-x.
2001.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
American Journal of Reproductive Immunology
Gleicher, Norbert
Kopenhagen: Munksgaard
Podaci o skupu
VIII International Congress of Reproductive Immunology.
poster
02.07.2001-06.07.2001
Opatija, Hrvatska