engleski

Production of cellulase enzyme system by Trichoderma reesei RUT C-30

The soft-rot fungus Trichoderma reesei RUT C-30 under optimal conditions produces high yields of extracellular cellulase enzyme system which hydrolyses macromolecular insoluble cellulose to monosaccharide glucose. The fermentation and the enzyme production process were performed in a rotating shaker at 350 rpm, 30 °C and pH of 5.5 &#8211 ; 6.0, with different substrate concentrations. As carbon sources were used: solka floc (100% cellulose), milk whey powder from the cheese industry and old corrugated cardboard paper. The process run in batch and fed-batch mode of operation and the FPU and ß-glucosidase activities of cellulases were determined. In the first experiment fermentation was performed with optimal sugar concentration of substrates: 10 mgml-1. The second fermentation was performed with the reduced sugar concentration of whey powder: 5 mgml-1. The third fermentation run in fed-batch mode of operation with the initial sugar concentration of 5 mgml-1 of whey powder for 48 hours and then two different substrates were added: solka floc and old corrugated cardboard paper, both with the sugar concentration of 5 mgml-1. With the same concentrations of less expensive starting material, higher enzyme activity results were achieved in the fed-batch mode of operation. The comparison of results for different substrates led to the conclusion that expensive pure cellulose can be successfully replaced with low walue industrial by-products as substrates for enzyme production and make the enzyme production process, as such, more economical.

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