engleski

Early response of white cabbage (Brassica oleracea var. capitata) to increased salinity: transcriptomic, hormonal and metabolic status

Soil salinization is a major agricultural problem, which impairs crops' growth, yields and quality. To meet problems caused by salinity, knowledge of major crops' tolerance levels and mechanisms is clearly important. Currently, most Brassica crop species are classified as moderately salt tolerant. To assist efforts to elucidate mechanisms responsible for salt sensitivity in Brassicaceae, Brassica crop with global economic importance: white cabbage (Brassica oleracea var. capitata) was used in this study. Initial, early salinity response (within 24 hours) of cabbage upon 200 mM NaCl was observed on transcriptome and hormonal level. Transcriptome analysis revealed changes in expression levels of genes involved in salt, osmotic and oxidative stress defense. Moreover, differences in transcript levels of genes regulating signaling and homeostasis of main stress hormones: abscisic acid (ABA), salicylic acid (SA) and jasmonates (JAs) and growth regulator indole-3-acetic acid (IAA) occurred. Genes involved in biosynthesis of glucosinolates, specialized metabolites of Brassicaceae family, were triggered by salinity too. To determine phytohormone and glucosinolates levels upon stress conditions, UHPLC-MS/MS analysis of target analytes was conducted. Taken together, acquired results provided insight in hormonal crosstalk, metabolite status and underlined mechanism of salinity response in white cabbage.

abiotic stress ; Brassicaceae ; climate changes ; transcriptomic ; metabolomic

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