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Hempseed and flaxseed protein hydrolysates as cell culture media supplements

Oil-cakes of hempseed and flaxseed contain about 30% proteins, and as such can be processed into nutrient supplement for animal cell cultivation. The main objective of the proposed study is to determine effects of flaxseed oil-cake protein isolates, hempseed oil-cake protein hydrolysates and hydrolysate peptide fractions on growth and productivity of IgG producing CHO cell line. Our preliminary results indicate that oil-cake protein hydrolysates cannot entirely replace the serum in culture media but they show potential of being a beneficial culture media supplement.

hempseed ; flaxseed ; protein hydrolysates ; animal cells ; medium

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