engleski

Chromosome abnormalities in neuroblastoma: cytogenetic and FISH analysis

Neuroblastoma is one of the most common solid tumors in children. Cytogenetic analysis revealed recurrent chromosome aberrations. The chromosome rearrangements important in tumor evolution are near-diploid or tetraploid chromosome number, terminal deletions of the short arm of chromosome No. 1, double minutes, homogeneously staining regions and amplification of the N-myc protooncogene. These genomic imbalances contribute to the tumor phenotype and carry useful prognostic information. In this report the results of cytogenetic analysis in 10 children with neuroblastoma are reported. The aim of this study is to identify numerical and structural aberrations and determine the frequency and types of acquired genomic abnormalities in our group of children with neuroblastoma. Cytogenetic investigation was performed on slides obtained by direct method of tumor tissue treatment. GTG and CBG-banding method were used for chromosome identification. FISH analysis was carried out using locus specific and chromosome specific centromeric probes (Vysis). The analysis of malignant cells revealed chromosome abnormalities in 9 cases, while in one tumor normal diploid karyotype was identified. Most tumor presented near-diploid or tetraploid chromosome number while one was near-triploid. Aberrations of chromosome No. 1 were the most frequent clonal rearrangements including translocations, terminal deletions, duplication and isochromosome. N-myc amplification was detected by cytogenetic and FISH analysis in one patient. This study confirms cytogenetic heterogeneity of neuroblastoma, and advantages of FISH, especially interphase FISH in the detection of genomic changes in neuroblastoma.

Neuroblastoma; cytogenetics; FISH

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