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Flavonoids of Guiera senegalensis J.F. GMEL.- Thin-Layer Chromatography and Numerical Methods

The efficiency of seven thin-layer chromatographic systems for separating nine flavonoids isolated from Guiera senegalensis J.F.GMEL. ( Combretaceae ) was investigated. For this purpose, three mathematical techniques were applied. The first technique was the calculation of the information content derived from Shannon's equation for each chromatographic system. In the second technique, the discriminating power of the systems was measured individually and in various combinations. The third technique classifies chromatographic system according to clusters. The classification was carried out by the numerical taxonomy methods. The most suitable chromaographic systems for the separation of the investigated flavonoids are: ethyl acetate: ethylmethylketon: formic acid:water, 60:15:3:2, and ethyl acetate: formic acid: acetic acid: water, 100:11:11:27.

Guiera senegalensis J.F.GMEL; Numerical Taxonomy; Discriminating Power; Information Contents; Clusters; Dendrogram

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