engleski

Differential gene expression related to developmental processes in plant tissue culture

The aim of the present study was to contribute the understanding of plant development and morphogeneses. In vitro cultures of horseradish (Armoracia lapathifolia Gilib.), sugar beet (Beta vulgaris L.) and cactus (Mammillaria gracillis Pfeiff.) tissues, were shown to be convenient systems to study cell differentiation and dedifferentiation (habituation and tumorigeneses). Specific developmental processes were triggered by intrinsic cellular factors and environmental conditions without the addition of exogenous growth regulators. Changes in gene activation programs were studied at the level of cellular and extracellular proteins and isoenzymes of peroxidase and esterase. Proteins were separated by means of 1-D and 2-D electrophoresis and silver-stained or characterised by Westernblotting for HSP70 (heat shock protein) and by lectins ConA, UEA, RCA120 for cellular and extracellular glycoproteins. Electrophoretic patterns of isoesterase and isoperoxidase showed tissue specificity and a correlation with the level of tissue organisation. Extracellular protein and even more glycoprotein patterns were tissue specific. The 2-D electrophoresis revealed several tissue specific protein groups, which should be closer characterised.

gene expression

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