engleski

The surface antigen CD45R identifies a population of estrogen-regulated murine marrow cells that contain osteoclast precursors.

We examined the osteoclastogenic potential of murine bone marrow cells that were fractionated according to their expression of the surface antigen CD45R. Osteoclast-like cells (OCL) with many authentic osteoclast characteristics readily formed in purified CD45R+ murine bone marrow cell cultures after treatment with receptor activator of nuclear factor B ligand (RANKL) and M-CSF. Ovariectomy (Ovx) caused a 1.5- to 2-fold increase in OCL number in unfractionated and CD45R+ murine bone marrow cell cultures without affecting OCL formation in CD45R- marrow cells. Limiting dilution assays confirmed that Ovx caused an increase in osteoclast precursor cell number in CD45R+ but not CD45R- cells. Mice deficient in the type 1 IL-1 receptor (IL-1R1 KO) do not lose bone mass after Ovx. We found that unfractionated, CD45R+, and CD45R- bone marrow cells from IL-1R1 KO mice showed no increase in OCL formation in vitro after Ovx. In both the wild-type (WT) and the IL-1R1 KO mice Ovx was associated with a 2-fold increase in pre-B-lymphocytes. About 1.3 - 3.5% of murine marrow cells expressed surface RANK (the receptor for RANKL) while about 11.9 - 15% of murine bone marrow cells expressed c-Fms (the receptor for M-CSF). There was little effect of Ovx on cells expressing either RANK or c-Fms. These results demonstrate that CD45R expression identifies a subset of murine bone marrow cells whose ability to form OCL in vivo is regulated by estrogen in WT but not IL-1R1 KO cells. The effects of estrogen on bone mass may be related to these responses.

B-lymphocytes; Bone marrow; Bone resorption; Flow cytometry; Ovariectomy; Type 1 IL-1 receptor knockout mice

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