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Genetic mapping in Miscanthus sinensis using 'offspring cross' mapping strategy

An 'offspring cross' mapping strategy was used in combination with the random amplified polymorphic DNA (RAPD) assay to construct the first genetic map of the species Miscanthus sinensis (2n = 2x = 38). The map is based on an outbred population of 89 individuals resulting from the cross between two fullsibs from a previously designed cross. The same proportion of bi-parental markers (heterozygous in both parents) and pseudo-testcross mono-parental markers (heterozygous in one parent and null in the other) have been obtained. A total of 383 RAPD markers were analyzed within the 89 F1 plants. Out of these markers, 257 were mapped into 28 linkage groups which spanned a total map length of around 1074.5 cM with an average density of 4.2 cM per marker. The contribution to the map was equal from both parents. The map provides a useful tool for genetic analyses of agronomically interesting characters in M. sinensis such as flowering, yield, plant height, stem diameter, and mineral constitution. The offspring cross mapping strategy is proposed to obtain a higher efficiency in developing integrated maps including both parents.

Miscanthus sinensis; RAPDs; genetic mapping; offspring cross

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