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Correlation between vimentin expression and nuclear grade in renal cell carcinoma

Aim: To analyze the correlation between the expression of vimentin and nuclear grade of renal cell carcinoma (RCC). Methods: In this study, tumor tissue from 20 patients was analyzed. There were 7 male and I 3 female patients with RCC conf ned to the kidney (pT I , pT2), ranging in age from 36-80 years (mean 60, 6 years).Thirteen tumors measured 5 cm in diameter and 7 were <5 cm.Two tumors were papillary and I 8 showed non-papillary pattern. Six tumors were composed of granular cells and I 4 of clear cells. Formalin fixed, paraffin-embedded tumortissue was cut at 5 Nm, deparaffnized, and stained with a monoclonal antibody to vimentin (Dako, Danemark).The intensity of immunostaining was graded semiquantitatively and denoted as (-) for no staining ; (+) for weak ; (++) for moderate ; and (+++) for strong reaction. Nuclear grade was determined according to Fuhrman et al. Am J Surg Pathol I 982 ; 6:655. Results: There were 4 tumors with nuclear grade I , 3 with grade 2, I I with grade 3, and 2 with nuclear grade 4. Histopathological examination showed vimentin expression in eight cases (40.0%), while I 2 tumors (60.0%) were negative. Positive reaction for vimentin was observed in all four tumors grade I , none of three tumors grade 2, four of I I tumors with grade 3, and in none of 2 tumors with nuclear grade 4. In our study there was no correlation between the expression of vimentin and nuclear grade of RCC. Conclusion: Our study shows a lack of correlation between the expression of vimentin and nuclear grade in renal cell carcinomas. Further studies on a larger series of tumors are obviously needed.

renal cell carcinoma; vimentin expression; nuclear grade

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