engleski

C-erbB-2 ONCOPROTEIN LEVELS IN SERUM AND TISSUE OF PATIENTS WITH COLORECTAL CARCINOMAS

We report our preliminary data concerning the determination of c-erbB-2 oncoprotein in senrm and tumours tissue of 38 patients with colorectal carcinomas. The patients were treated by surgery followed by chemotherapy. The serum levets of c-erbB-2 oncoprotein were measured by immunosorbent assay techniques (Oncogene Sciences). lmmunohistochemistry was performed using two different primary antibodies (Oncogene Sciences and DAKO). The alkaline phosphatase/antialkaline phosphatase and horse-radish peroxidase/antiperoxidase methods for investigation of the corresponding tumour tissue was used. lncreased serum c-erbB-2 oncoprotein was found in 23 patients. The extent of serum oncoprotein expression corresponded to the clinical stage of the tumour and to the immunostaining of the tumour tissue. lmmunohistochemical analysis of primary tumours showed strong positivity in one case, moderate positivity in nine cases, and slight positivity in 16 cases. Positive irnnttmostaining was observed in three cases in both primary tumours and lymph node metastasis. Both methods showed comparable results.The serum c-erbB-2 oncoprotein may be useful for monitoring the patients with colorectal carcinomas particularly in cases showing positive immunostaining of primary tumour. Further investigation of a larger series of patients with a long-term follow-up is obviously needed.

C-erbB-2 ONCOPROTEIN; COLORECTAL CARCINOMAS

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