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Gender differences in expression of rat renal OAT1 (CROSBI ID 739338)

Prilog sa skupa u časopisu | izvorni znanstveni rad

Burckhardt, Gerhard ; Ljubojević, Marija ; Herak-Kramberger, Carol M. ; Hagos, Yohannes ; Bahn, Andrew ; Endou, Hitoshi ; Sabolić, Ivan Gender differences in expression of rat renal OAT1 // Pflügers Archiv. 2003. str. S40-x

Podaci o odgovornosti

Burckhardt, Gerhard ; Ljubojević, Marija ; Herak-Kramberger, Carol M. ; Hagos, Yohannes ; Bahn, Andrew ; Endou, Hitoshi ; Sabolić, Ivan

engleski

Gender differences in expression of rat renal OAT1

The gender differences (GD) in the renal secretion of organic anions may be due to different expression levels of the organic anion transporter 1 (OAT1) in the basolateral membrane (BLM) of the S2 proximal tubule segment. By using immunofluorescence cytochemistry in cryosections of fixed renal tissue and immunoblotting of isolated renal cortical BLM and total cell membranes, we studied localization and the abundance of OAT1 in adult and prepubertal male (M) and female (F) rats, as well as an effect of gonadectomy and steroid replacement therapy. The expression of OAT1 protein in the S2 segment was stronger in M than in F. In prepubertal rats, OAT1 abundance was low and exhibited no GD. In adult M, the abundance of OAT1 decreased by 45% two weeks after castration, whereas daily s.c. treatment of castrated M with testosterone, estradiol, or progesterone (2.5 mg/kg b.m. each) for 8 days resulted in a complete restitution, further (88%) depression, or overexpression (22%) of OAT1, respectively. In F rats, ovariectomy had no effect, but additional treatment with estradiol caused a strong decrease (73%) of OAT1. Immunoblotting data completely matched the findings by immunofluorescence. We conclude that GD in renal OAT1 expression are due to stimulation by androgens and progesterone, and inhibition by estrogens.

organic anion transporters; sex differences; testosterone; estrogen

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Podaci o prilogu

S40-x.

2003.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Pflügers Archiv

0031-6768

Podaci o skupu

Nepoznat skup

ostalo

29.02.1904-29.02.2096

Povezanost rada

Temeljne medicinske znanosti, Kliničke medicinske znanosti

Indeksiranost