engleski

Corticosteroid-impairment of haeling and gastric pentadecapeptide BPC-157 creams in burned mice

The amelioration of corticosteroid-imparment of healing by a atable gastropentadecapeptide BPC-157 ( GEPPPGKPADDAGLV, M(w)1419, currently in early clinical trials for inflammatory bowel disease) was studied in thermally injured mice.Its effects on corticosteroid impaired healing of deep partial skin thiuckness burns, and burn-gastric lesions were investigated.Male NMRI-Hannover mice( sacrificed at 1-3, 7, 14 and 21 days following burning 20% of total burn area at the back ( open flame for 7s) received intraperitoneally( per kg bw) 6alpha-methylprednisolone( Depomedrol, 1.0 or 10.0mg) or an equal volume of saline ( 5.0ml), once daily, first application 30 min after injury, last 24h before sacrifice.The injuryas subsequently treated by topical application of a thin layer of pentadecapeptide BPC-157 cream at three different levels a neutral cream of no treatment.Pentadecapeptide BPC-157 consistently improved given burn healing( both microscopical and tensionmetry assessment) and countreracted corticosteroid-impairment of burn healing.In burn-gastric lesion investigation of the effects of BPC showed an anti-ulcer effect of its own in burned non-corticosteroid-treated mice and potentiated the anti-ulcer effect observed in 6 alpha-methylprednisolone-treated mice.Pentadecapeptide BPc-157 inhibited corticosteroid immunosuppression.In vitro, in spleenic cells assessment, animals( sacrificed at day 21) treated with 6alpha-methylprednisolone 1mgshowed decreased reactivity to nitrogen in comparation with control, healthyy animals, while the addition of BPC-157 ( 1micro/ g cream) returned cell reactivity to values noted in control healthy animals.

Corticosteroid-impairment; Healing; Gastric pentadecapeptide; BPC-157

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