engleski

Examination of cytotoxicity and mutagenicity of AH 26 and AH Plus sealers

Aim was to study in vitro the cytotoxic and mutagenic effects of AH26 and AH Plus. Cytotoxic effects on Chinese hamster V79 cells were determined by counting viable cells following incubation with eluations of AH26 and AH Plus. In one set of experiments the materials were mixed. Set for 1 h and then eluted with dimethylsulphoxide (DMSO) for 1 h. 24 h and 7 days. In the other set. AH26 and AH Plus were mixed and set for 1 h. 24 h and 7 days in physiological saline then crushed and eluted in DMSO for 24 h. The cytotoxic effects of these eluates were evaluated. Three concentrations were chosen to examine the mutagenic effects of AH26 and AHlus: 5.57.16.7 and 55.7 f.lg mL -1. The structural chromo- somal aberration analysis and micronucleus test were performed on human Iymphocytes according to stan- dard procedures. Dose-response curves of cell survival were obtained. Both materials were shown to be cytotoxic in doses Iarger than 55.7 I.1g mL -1, except for AH26. AH Plus was also shown to be toxic in concentrations of 16.7 I.1g mL -1, except after 7 days setting time. NeitherAH26 nor AH Plus induced a significant increase of chromosomal aberrations or micronuclei induction at any setting time or concentration. There was no mutagenicity found for AH26 and AH Plus on human Iymphocytes in highly controlled conditions in vitro.

AH26 ; AHPlus ; chromosomal aberrations ; human lymphocytes ; micronucleus ; V 79

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