engleski

Application of the Alkaline Comet Assay in Biomonitoring of Medical Personnel Occupationally Exposed to Ionizing Radiation

The alkaline comet assay was selected as a biomarker of exposure to evaluate the ongoing exposure to ionizing radiation in medical workers occupationally exposed to ionizing radiation and corresponding non-exposed control subjects. The primary DNA damage was evaluated by measuring the extent of DNA migration in peripheral blood leukocytes. The interindividual differences in DNA damage between exposed subjects were also assessed by comparison of their dosimeter readings. It was found that medical workers who were occupationally exposed to ionizing I radiation for different periods of time showed significantly increased levels of DNA darnage compared with control subjects. There was a significant correlation between the severity of DNA damage and duration of exposure. However, no significant correlation was found between the age of all subjects and DNA damage. According to our results, the alkaline comet assay as a sensitive technique for the assessment of the DNA damage could be usefully applied in the regular health screening of workers occupationally exposed to ionizing radiation.

comet assay; occupational exposure; ionizing radiation

Rad je objavljen i u Knjizi sažetaka sa Skupa, str. 200-200.