engleski

Two different clean-up procedures for HPLC analysis of ochratoxin A in urine

This paper describes two different procedures of ochratoxin A (OTA) extraction from urine samples. A procedure with acidic chloroform-methanol mixture, followed by solid-phase extraction (SPE) clean-up was compared with extraction on commercial Chem Elut columns with a chloroform-formic acid mixture. The recovery of OTA using the procedure with silica gel columns was 82% with a RSD <8.4%, and the detection and quantitation limit were 0.5 ng OTA/ml and 1.5 ng OTA/ml. The recovery of OTA in the second procedure with urine samples purified only on commercial Chem Elut columns was 95% with RSD <4.0%, and detection and quantitation limit of 0.3 ng/ml and 0.9 ng/ml, respectively. Both procedures of OTA extraction effectively eliminate interfering substances and give reliable and repeatable results. However, the procedure with Chem Elut columns gave higher recovery and lower detection and quantification limit. It was successfully applied in determining OTA in human urine samples.

ochratoxin A; urine; silica gel columns; Chem Elut columns; HPLC

nije evidentirano