engleski

Expression of bone morphogenic proteins in acute promyelocytic before and after combined all treans-retinoic acid and cytotoxic treatment

Bone morphogenetic proteins (BMPs) belong to the TGFb-superfamily of molecules and have important regulatory roles in embryonic morphogenesis and development, as well as in cell growth and differentiation in various tissues, including hematopoietic. In our study, we investigated the dynamics of bone morphogenetic protein and their receptor mRNA expression in relation to combined treatment with all trans-retinoic acid and chemotherapy in four patients with acute promyelocytic leukemia (APL). RNA was extracted after minimal manipulation from the bone marrow at the time of diagnosis and at the time of hematological marrow evaluation required by the treatment protocol. RT-PCR assay was designed to detect the transcripts of BMP-2, -4, and -7, as well as of BMP receptors IA, IB, and II. At diagnosis, we found strong expression of BMP-2, -4, and -7, and their receptors RIA, RIB, and RII, parallel to the expression of PML/RARa fusion gene transcripts. Therapeutic clearance of the tumor molecular marker corresponded to the reduction of BMP expression, suggesting the possible role of BMPs as markers of the minimal residual disease in APL.

acute promyelocytic leukemia; bone marrow; retinoic acid; bone morphogenetic proteins

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