engleski

Ultratraces of carotenes in tomato purées: HPLC-TLS Study

The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and ß-carotene left in tomato purees. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and 1.1 ng/ml lycopene and ß- carotene in purée extracts, respectively. Total concentration of ß-carotene and lycopene (varying from 3 to 170 ng/g) in the examined tomato purées may serve as an indicator of the carotene-specific antioxidative capacity of these products. Although conventional spectrophotometry can be used to rapidly assess the quality of products derived from tomatoes, a highly sensitive and selective method such as HPLC-TLS is needed for reliable analyses of samples such as, for example, those subjected to inappropriate storage and/or handling.

lycopene; ß-carotene; tomato purée; antioxidant capacity; HPLC-TLS

Rad je prezentiran na skupu 12th International Conference on Photoacoustic & Photothermal Phenomena, održanom od 24.-27.06.2002.g., Toronto, Kanada.