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Tumorski makrofagi u regulaciji angiogeneze invazivnog duktalnog raka dojke (CROSBI ID 337884)

Ocjenski rad | doktorska disertacija

Valković, Toni Tumorski makrofagi u regulaciji angiogeneze invazivnog duktalnog raka dojke / Jonjić, Nives (mentor); Jonjić, Nives (neposredni voditelj). Rijeka, Medicinski fakultet u Rijeci, . 2002

Podaci o odgovornosti

Valković, Toni

Jonjić, Nives

Jonjić, Nives

hrvatski

Tumorski makrofagi u regulaciji angiogeneze invazivnog duktalnog raka dojke

Objectives: the aims of this investigation were to determine infiltrations of Tumor Associated Machrophages (TAM) as well as expresion of Vascular Endothelial Growth Factor (VEGF) and basic Fibroblast Growth Factor (bFGF) in the tumr parenchymal of ductal invasive breast carcinomas NOS and correlate these parameters to each other and wth maximal microvessel density (MVD). Also, the expresion of Monocite Chemotactic Protein - 1 (MCP - 1) in the tumr cells of this kind of breast cancer was determined and compared with macrophage infiltrations and above - mentioned parameters. Finally, the tumor´s capability to produce mentioned chemokine was, also, analysed by determination the level of messenger - ribonucleic acid (mRNA) for MCP - 1. Material and methods: 97 ductal invasive breast carcinomas NOS were analysed by immunohistochemistry using "avidin - biotin" method and macrophage infiltration, MVD, expression of MCP - 1, VEGF and FGF (63 samples of breast carcinoma were analysed) in the tumor cells were determined. The expression of messenger - ribonucleic acid for MCP - 1 was examined by reverse transcriptase - polymerase chain reaction (RT - PCR) in the 8 samples of breast carcinomas and control normal breast tissues. Statistical analysis was performed by the personal computer using chi square test, t - test and "one way" analysis of variance. Results: statistical analysis pointed that breast carcinomas with only indivudual stromal macrophages had significantly decreased MVD in the comparation with focally and diffusely infiltrated tumros (p = 0, 005). Also, postive corelations between parenchymal expression of VEGF and MVD (p < 0, 001), macrophage infiltrations (p = 0, 003) and expression of bFGF (p = 0, 016) were found. The corelation between expession of MCP - 1 and macrophages infiltrations was not found and the results of the polymerase chain reaction pointed that breast carcinomas, as well as normal breast tissue can expressed messenger ribonucleic acid for MCP - 1. Conclusion: in the ductal invasive breast carcinomas NOS, TAM promote angiogenesis as well as VEGF produced by tumor parenchymal. Also, this angiogenic factor has chemotactic influence on macrophages. bFGF has no angiogenic properties in this kind of breast cancer and the expression of this angiogenic factor and VEGF can be achieved by the same regulatory mechanisms.

duktalni invazivni karcinom dojke NOS; tumorski makrofagi; gustoća malih krvnih žila; monocitna kemotaktična bjelančevina -1; vaskularni endotelni čimbenik rasta; bazični fibroblastni čimbenik rasta; angiogeneza; kemotaksija

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Podaci o izdanju

124

17.09.2002.

obranjeno

Podaci o ustanovi koja je dodijelila akademski stupanj

Medicinski fakultet u Rijeci

Rijeka

Povezanost rada

Kliničke medicinske znanosti