engleski

Identification of giant and oligovesicular vesicles using polarizing microscopy

It has been found that in certain surfactant single (surfactants that are strongly hydrophylic and have large hydrophobic groups like double chained surfactants) and complex systems (mixtures of oppositely charged surfactants, mixtures of oppositelly charged surfactants and biopolymers, etc.) the vesicular structures form spontaneously and are long-lived. A question inevitably arises with all vesicle systems: are the vesicle unilamellar, multilammelar or oligovesicular. Usually they can be differentiated by freeze-fracture electron microscopy, which is expensive and a time consuming method. Vesicles of sufficient size to observe under the light microscope (so-called giant vesicles) are receiving heightened attention. It should be noted that phase-contrast light microscopy can, under ideal condition, differentiate between unilamellar, multilamellar or oligovesicular vesicles. In this work we shall show how unilamellar and oligovesicular vesicles can be identified by using crossed polarizers.

vesicles; polarizing microscopy

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