Differentiation of animal virus strains by restriction endonuclease analysis (CROSBI ID 495927)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Lojkić, Ivana ; Biđin, Zdenko ; Grce, Magdalena ; Husnjak, Koraljka ; Pokrić, Biserka
engleski
Differentiation of animal virus strains by restriction endonuclease analysis
The restriction enzyme (RE) analysis of the reverse transcription (RT)/polymerase chain reaction (PCR) products of viral genome segments is proposed as a method for the differentiation of the field isolates of animal viruses. The accuracy of the method was tested on the infectious bursal disease viruses (IBDV) responsible for the outbreak of the disease among chicken in Croatia during a 5 year period. The analysis was conducted with the 422 bp hypervariable region of the VP2 gene. The RT/PCR products were digested using the restriction enzymes CfoI, SacI, SspI, StuI and TaqI. The knowledge of the nucleotide sequence recognized by the endonuclease, the literature and the GenBank nucleotide data for the analyzed VP2 gene region, as well as the size of the fragments obtained by the endonuclease digestion, enabled the determination of the nucleotide position corresponding to a restriction site. The differentiation of the analysed IBDV species was obtained by comparing the position of the restriction sites in analysed IBDV species with the data reported elsewhere. The RE-RT/PCR analyses showed that 98% of IBDV positive tissue samples were infected with very virulent IBDV strains. This result agreed and explained a high rate of chicken morbidity and mortality in the period of sample collection.
infectious bursal disease virus; RT-PCR; restriction endonuclease
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Podaci o prilogu
49-x.
2003.
objavljeno
Podaci o matičnoj publikaciji
2nd scientific symposium with international participation - 45 years of molecular biology in Croatia and 50 years of double helix
Ambirović Ristov, Andreja ; Brozović, Anamaria
Zagreb:
Podaci o skupu
45 years of molecular biology in Croatia and 50 years of double helix
poster
20.11.2003-21.11.2003
Zagreb, Hrvatska