Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Differentiation of animal virus strains by restriction endonuclease analysis (CROSBI ID 495927)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Lojkić, Ivana ; Biđin, Zdenko ; Grce, Magdalena ; Husnjak, Koraljka ; Pokrić, Biserka Differentiation of animal virus strains by restriction endonuclease analysis // 2nd scientific symposium with international participation - 45 years of molecular biology in Croatia and 50 years of double helix / Ambirović Ristov, Andreja ; Brozović, Anamaria (ur.). Zagreb, 2003. str. 49-x

Podaci o odgovornosti

Lojkić, Ivana ; Biđin, Zdenko ; Grce, Magdalena ; Husnjak, Koraljka ; Pokrić, Biserka

engleski

Differentiation of animal virus strains by restriction endonuclease analysis

The restriction enzyme (RE) analysis of the reverse transcription (RT)/polymerase chain reaction (PCR) products of viral genome segments is proposed as a method for the differentiation of the field isolates of animal viruses. The accuracy of the method was tested on the infectious bursal disease viruses (IBDV) responsible for the outbreak of the disease among chicken in Croatia during a 5 year period. The analysis was conducted with the 422 bp hypervariable region of the VP2 gene. The RT/PCR products were digested using the restriction enzymes CfoI, SacI, SspI, StuI and TaqI. The knowledge of the nucleotide sequence recognized by the endonuclease, the literature and the GenBank nucleotide data for the analyzed VP2 gene region, as well as the size of the fragments obtained by the endonuclease digestion, enabled the determination of the nucleotide position corresponding to a restriction site. The differentiation of the analysed IBDV species was obtained by comparing the position of the restriction sites in analysed IBDV species with the data reported elsewhere. The RE-RT/PCR analyses showed that 98% of IBDV positive tissue samples were infected with very virulent IBDV strains. This result agreed and explained a high rate of chicken morbidity and mortality in the period of sample collection.

infectious bursal disease virus; RT-PCR; restriction endonuclease

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

49-x.

2003.

objavljeno

Podaci o matičnoj publikaciji

2nd scientific symposium with international participation - 45 years of molecular biology in Croatia and 50 years of double helix

Ambirović Ristov, Andreja ; Brozović, Anamaria

Zagreb:

Podaci o skupu

45 years of molecular biology in Croatia and 50 years of double helix

poster

20.11.2003-21.11.2003

Zagreb, Hrvatska

Povezanost rada

Veterinarska medicina, Biologija